DM1 ADC EIA Kit DEIABL311 from Creative Diagnostics

Product Specs
ItemDM1 ADC EIA Kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberDEIABL311
Quantity96T
Sample Typetissue cell cultures, serum
SensitivityThe analytical sensitivity (LLOD) of this DM1 ADC EIA as determined by the 2 times calibrator deviation below the mean of B0 on 8 duplicate determinations of zero calibrator (B0) is approximately 0.024 µg/mL
ReactivityHuman, Rat, Mouse, Primate
ComponentsThis test kit must be stored at 2 - 8°C upon receipt. For the expiration date of the kit refer to the label on the kit box. All components are stable until this expiration date.
Prior to use allow all reagents to come to room temperature.
Regents from different kit lot numbers should not be combined or interchanged.
1. Anti-DM1 Antibody Coated Microplate
One microplate with twelve by eight strips (96 wells total) coated with anti-DM1 antibody. The plate is framed and sealed in a foil zipper bag with a desiccant. This reagent should be stored at 2 - 8°C and is stable until the expiration date on the kit box.
2. HRP Conjugated DM1
One vial containing 3 mL of ready to use HRP labeled DM1 in a stabilized protein matrix. This reagent should be stored at 2 - 8°C and is stable until the expiration date on the kit box.
3. ELISA Wash Concentrate
One bottle containing 30 mL of 30-fold concentrate. Before use the contents must be diluted with 870 mL of demineralized water and mixed well. Upon dilution, this yields a working wash solution containing a surfactant in phosphate buffered saline with a non-azide, non-mercury preservative. The diluted wash solution may be stored at room temperature and is stable until the expiration date on the kit box.
4. ELISA HRP Substrate
One bottle containing 15 mL of tetramethylbenzidine (TMB) with hydrogen peroxide. This reagent should be stored at 2 - 8°C and is stable until the expiration date on the kit box.
5. ELISA Stop Solution
One bottle containing 15 mL of stop solution. This reagent may be stored at 2 - 8°C or room temperature and is stable until the expiration date on the kit box.
6. Antibody-DM1 Conjugated Calibrator Zero (Cat.No.CD41812)
One vial containing 30 mL calibrator zero (CD41812). This reagent is used for diluting the calibration stock t make assay calibrators, as well as for diluting test samples. This reagent should be stored at 2-8°C and is stable until the expiration date on the kit box.
7. Antibody-DM1 Conjugated Calibration Stock (Cat. No. CD41813) - Not provided in the kit (optional)
One vial (CD41813) containing the calibration stock of antibodyDM1-conjugate in a lyophilized (0.5 mL) serum based matrix with a non-azide preservative. Refer to the vial for exact concentration of the standard. This reagent should be stored at 2 - 8°C and is stable until the expiration date on the kit box
PrecisionThe intra-assay precision was validated by measuring three calibrators (L3, L5 and L7) in six replicate determinations. The CV% is 6.8%, 6.2% and 8.9%
ApplicationsQuantitative

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Creative Diagnostics

45-1 Ramsey Road

Shirley, N 11967

United States

Phone: 1-631-624-4882 (USA)
44-161-818-6441 (Europe)

Email: info@creative-diagnostics.com

Company Profile

Website: https://www.creative-diagnostics.com/

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DM1 ADC EIA Kit from Creative Diagnostics

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash

DM1 ADC EIA Kit from Creative Diagnostics

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